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Results and discussion 3. Conclusion We optiarc dvd -rw nd-3570a developed a high-throughput imaging cell-based assay platform based on integration of time-stretch imaging and high-speed spinning DVD. Acknowledgment We thank Shing Chan for providing the human buffy coat.
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References and links 1. Boutros M. Natasha I. Cancer 14 9— Feng Y. Drug Discov.
Time-stretch microscopy on a DVD for high-throughput imaging cell-based assay
Lang P. Goda K.
Photonics 7 2 In practice, such an enormous size of image data can only be handled when the imaging system is integrated with a high-throughput data acquisition platform, e. Compared to time-stretch imaging, automated microscopy, which is a widely adopted tool for image-based high-content screening [ optiarc dvd -rw nd-3570a ], runs short of sufficient imaging throughput.
This is primarily limited by the frame rate achieved by the current camera technologies, which hinders fast automated specimen stage scanning. Moreover, essentially all classical optical microscopes have a fundamental space-bandwidth-product limit of optical imaging, in which high resolution imaging compromises the FOV, and thus throughput [ 26 ]. More significantly, we have demonstrated that this system is compatible with both adherent cell culture and biochemically-specific cell capture assay formats — further enlarging the scope of time-stretch imaging, which has predominately been limited to the suspension-cell format [ 7optiarc dvd -rw nd-3570a — 1220 ].
Furthermore, the single-cell phenotypes extracted from the time-stretch images are no longer restricted primarily to biophysical properties, but also the molecular signatures e. We also note that this spinning-disc assay platform can be compatible with not only bright-field and quantitative phase imaging, but also fluorescence imaging.
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For the sake of demonstration, up to 8 assay sites have been incorporated on the DVD-based substrate in the current optiarc dvd -rw nd-3570a. The planar platform also makes it straightforward to combine the ultrafast imaging capability with the established centrifugal microfluidic technologies for active fluid control, e. Such assay integration could allow more advanced assay functionalities and applications.
As a result, time-stretch spinning imaging cell-based assay could hold promise for addressing the unmet need for scaling both optiarc dvd -rw nd-3570a measurement throughput as well as content in many applications, e. Author information Article notes Copyright and License information Disclaimer. This article has been cited by other articles in PMC. Abstract Cell-based assay based on time-stretch imaging is recognized to be well-suited for high-throughput phenotypic screening.
OCIS codes: Introduction Accessing detailed spatial information of cells, microscopy allows high-content cell-based phenotypic assay, but at a compromised measurement throughput [ 12 ]. Materials and methods 2. Open in a separate window.
Results and discussion 3. Conclusion We have developed a high-throughput imaging cell-based assay platform based on integration of time-stretch imaging and high-speed spinning DVD. Acknowledgment We thank Shing Chan for providing the human buffy coat. References and links 1. We now have possibly not encountered any sort of situations for the duration of a lot of our screening; optiarc dvd -rw nd-3570a deep freeze, failure and appear problems. Wonder if I should swap drives.
I can probably live without the 16X capability though. Posted 17 July - PM. Ramachandraiah H.
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Methods 12 3— Zangle T. Methods 11 12— I optiarc dvd -rw nd-3570a a Sony-Nec Optiarc NDA that some day stop reading my DVDs media (it see like a blank DVD media); CDs media reading works fine; also i optiarc dvd -rw nd-3570a. On a Precision workstations, using Roxio EMC 9 and now EMC 10 (as well as other software) on XP SP2, I'm having problems with the DVD's that this.